Published 2023

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Publication details

Journal : Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy , vol. 301 , p. 1–8 , 2023

International Standard Numbers :
Printed : 1386-1425
Electronic : 1873-3557

Publication type : Academic article

Contributors : Kristoffersen, Kenneth Aase; Måge, Ingrid; Wubshet, Sileshi Gizachew; Böcker, Ulrike; Dankel, Elin Katinka Riiser; Lislelid, Andreas; Rønningen, Mats Aksnes; Afseth, Nils Kristian

Research areas

Bioprocessing

Quality and measurement methods

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Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no

Summary

Fourier transform infrared spectroscopy (FTIR) is a powerful analytical tool that has been used for protein and peptide characterization for decades. In the present study, the objective was to investigate if FTIR can be used to predict collagen content in hydrolyzed protein samples. All samples were obtained from enzymatic protein hydrolysis (EPH) of poultry by-products providing a span in collagen content from 0.3% to 37.9% (dry weight), and the FTIR analysis was performed using dry film FTIR. Since nonlinear effects were revealed by calibration using standard partial least squares (PLS) regression, Hierarchical Cluster-based PLS (HC-PLS) calibration models were constructed. The HC-PLS model provided a low prediction error when validated using an independent test set (RMSE = 3.3% collagen), while validation using real industrial samples also showed satisfying results (RMSE = 3.2%). The results corresponded well with previously published FTIR-based studies of collagen, and characteristic spectral features for collagen were well identified in the regression models. Covariance between collagen content and other EPH related processing parameters could also be ruled out in the regression models. To the authors' knowledge, this is the first time that collagen content has been systematically studied in solutions of hydrolysed proteins using FTIR. This is also one of few examples where FTIR is successfully used to quantify protein composition. The dry-film FTIR approach presented in the study is expected to be an important tool in the growing industrial segment that is based on sustainable utilization of collagen-rich biomass.

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