Published 2023

Read in Norwegian

Publication details

Journal : Microorganisms , vol. 11 , p. 1–16–15 , 2023

Publisher : MDPI

International Standard Numbers :
Printed : 2076-2607
Electronic : 2076-2607

Publication type : Academic article

Contributors : Vasiliauskaite, Agne; Mileriene, Justina; Kasparaviciene, Beatrice; Aleksandrovas, Elvidas; Songisepp, Epp; Rud, Ida; Axelsson, Lars; Muizniece-Brasava, Sandra; Ciprovica, Inga; Paskevicius, Algimantas; Aksomaitiene, Jurgita; Gabinaitiene, Ausra; Uljanovas, Dainius; Baliukoniene, Violeta; Lutter, Liis; Malakauskas, Mindaugas; Serniene, Loreta

Issue : 3

Research areas


Raw material knowledge

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian


The demand for healthy foods without artificial food additives is constantly increasing. Hence, natural food preservation methods using bioprotective cultures could be an alternative to chemical preservatives. Thus, the main purpose of this work was to screen the indigenous lactobacilli isolated from fermented cow milk for their safety and antifungal activity to select the safe strain with the strongest fungicidal properties for the development of bioprotective acid whey protein concentrate (AWPC) based fermentates and their coatings intended for fresh cheese quality maintenance. Therefore, 12 lactobacilli strains were isolated and identified from raw fermented cow milk as protective cultures. The safety of the stains was determined by applying antibiotic susceptibility, haemolytic and enzymatic evaluation. Only one strain, Lacticaseibacillus paracasei A11, met all safety requirements and demonstrated a broad spectrum of antifungal activity in vitro. The strain was cultivated in AWPC for 48 h and grew well (biomass yield 8 log10 cfu mL−1). L. paracasei A11 AWPC fermentate was used as a vehicle for protective culture in the development of pectin-AWPC-based edible coating. Both the fermentate and coating were tested for their antimicrobial properties on fresh acid-curd cheese. Coating with L. paracasei A11 strain reduced yeast and mould counts by 1.0–1.5 log10 cfu mL−1 (p ≤ 0.001) during cheese storage (14 days), simultaneously preserving its flavour and prolonging the shelf life for six days.


Topics associated with the publication