Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract
Publication details
Journal : PLOS ONE , vol. 16 , p. 1–14 , 2021
International Standard Numbers
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Electronic
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1932-6203
Publication type : Academic article
Issue : 2
Links
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DOI
:
doi.org/10.1371/journal.pone.0...
ARKIV
:
hdl.handle.net/11250/2783611
Research areas
Quality and measurement methods
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Summary
Article Authors Metrics Comments Media Coverage Abstract 1. Introduction 2. Materials and methods 3. Results and discussion 4. Conclusion Supporting information References Reader Comments (0) Figures Abstract In this work, a new magnetic ligand fishing probe for discovery of DPP-IV inhibitory ligands was developed and it was tested as a proof of concept on the fruit extract of Vaccinium vitis-idaea (lingonberry). The ligands were shown to have appreciable dipeptidyl peptidase IV (DPP-IV) inhibitory activity (IC50: 31.8 μg mL-1).) Inhibition of DPP-IV is a well-known therapeutic approach for management of type 2 diabetes (T2D). DPP-IV was successfully immobilized onto magnetic beads and was shown to retain its catalytic activity and selectivity over a model mixture. A total of four ligands were successfully fished out and identified as cyanidin-3-galactoside (2), cyanidin-3-arabinoside (3), proanthocynidin A (4), and 10-carboxyl-pyranopeonidin 3-O-(6″-O-p-coumaroyl)-glucoside (5) using HPLC/HRMS.