Published 2015

Read in Norwegian

Publication details

Journal : Carbohydrate Polymers , vol. 124 , p. 254–264 , 2015

Publisher : Elsevier

International Standard Numbers :
Printed : 0144-8617
Electronic : 1879-1344

Publication type : Academic article

Contributors : Rieder, Anne; Knutsen, Svein Halvor; Ulset, Ann-Sissel Teialeret; Christensen, Bjørn E.; Andersson, Roger; Mikkelson, Atte; Tuomainen, Päivi; Maina, Ndegwa; Ballance, Simon

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian


Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10–500 × 103 g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.