Investigation of the Effectiveness of Barrier Layers to Inhibit Mutagenic Effects of Recycled LDPE Films, Using a Miniaturized Ames Test and GC-MS Analysis
Publication details
Journal : Recycling , vol. 9 , p. 1–20–0 , 2024
Publisher : MDPI
International Standard Numbers
:
Printed
:
2313-4321
Publication type : Academic article
Issue : 4
Links
:
ARKIV
:
hdl.handle.net/11250/3157499
DOI
:
doi.org/10.3390/recycling90400...
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Kjetil Aune
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Summary
To fulfil the European Green Deal targets and implement a circular economy, there is an urgent need to increase recycling rates of packaging materials. However, before recycled materials can be used in food contact applications, they must meet high safety standards. According to the European Food Safety Authority (EFSA), a worst-case scenario must be applied and unknown substances must be evaluated as being potentially genotoxic. The Ames test, which detects direct DNA-reactive effects, together with chromatographic analysis is very promising to complement risk assessment. This study aims to evaluate the effectiveness of functional barriers in ten different samples, including virgin and recycled LDPE foils. FT-IR analysis did not show major differences between virgin and recycled films. Light microscopy revealed differences in quality and an increased number of particles. GC-MS analysis detected and quantified 35 substances, including eight unknowns. Using a miniaturized version of the Ames test, four of ten
