Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells
Publication details
Journal : Food Microbiology , vol. 113 , p. 1–12 , 2023
International Standard Numbers
:
Printed
:
0740-0020
Electronic
:
1095-9998
Publication type : Academic article
Links
:
DOI
:
doi.org/10.1016/j.fm.2023.1042...
ARKIV
:
hdl.handle.net/11250/3068496
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Summary
Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different discharge power (26 and 36 W) and activation time (5 and 30 min). The inactivation of a three-strain Listeria monocytogenes cocktail in planktonic and biofilm state was evaluated. PAW generated at 36 W-30 min showed the lowest pH and the highest hydrogen peroxide, nitrates, nitrites contents and effectiveness against cells on planktonic state, resulting in 4.6 log reductions after a 15-min treatment. Although the antimicrobial activity in biofilms formed on stainless steel and on polystyrene was lower, increasing the exposure time to 30 min allowed an inactivation >4.5 log cycles. The mechanisms of action of PAW were investigated using chemical solutions that mimic its physico-chemical characteristics and also RNA-seq analysis. The main transcriptomic changes affected carbon metabolism, virulence and general stress response genes, with several overexpressed genes belonging to the cobalamin-dependent gene cluster.