Published 2021

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Publication details

Publisher : Nofima AS

International Standard Numbers :
Printed : 978-82-8296-680-1

Publication type : Nofima’s reports

Contributors : Heikintalo, Noora; Asbjørnsen, Bente

Series : Nofima rapportserie 16/2021

Year : 2021

Research areas

Feed development and nutrition

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian


The purpose of this project was to validate the method "NS-EN 12822: 2014" for the determination of vitamin E content (α-, β-, γ- and δ-tocopherols) in feed and feed ingredients. Dry samples are saponified with potassium hydroxide dissolved in ethanol. Vitamin E is extracted into the hexane-ethyl acetate phase which is transferred to the volumetric flask and diluted to the desired concentration. Vitamin E content is determined by high-performance liquid chromatography (HPLC, Agilent 1200) with fluorescence detection. The amount of α-, β-, γ- and δ-tocopherol is reported. The analysis method is called as BIOLAB A121 Tokoferolprofil in the internal quality system. It was performed a validation of accuracy, precision, linearity, measurement range and measurement uncertaint. Fish feed and feed ingredients (fish meal and oils) were selected as test material, because they are the most requested sample material for vitamin E analyze at Nofima Biolab. After a systematic review of results, the method is considered to be approved.


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