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Published 2003

Read in Norwegian

Publication details

Journal : Journal of Applied Microbiology , vol. 95 , p. 186–195 , 2003

International Standard Numbers :
Printed : 1364-5072
Electronic : 1365-2672

Publication type : Academic article

Contributors : Langsrud, Solveig; Møretrø, Trond; Sundheim, G.

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian


To determine if disinfecting footbaths in food industry were contaminated with bacteria and characterise some of the bacteria present.

Methods and results:
Bacterial strains were isolated from disinfecting footbaths containing TEGO 103G (amphoteric disinfectant) or TP-99 (alkylaminoacetate-based disinfectant) in five out of six dairy factories. Fourteen strains identified as Cedecea spp. by their fatty acid composition were further characterised. The reactions in the Rapid ID 32 E API analysis and 16S-rDNA-sequensing showed that all strains were Serratia marcescens. In contrary to Ser. marcescens ATCC 13880 the isolates from disinfecting footbaths were not killed (<5 log reduction) by the recommended in-use concentration of TEGO 103G, TEGO 51 or benzalkonium chloride. Survival and multiplication in tap water with in-use concentration of TEGO 103G was demonstrated for one of the strains. All strains were killed by the in-use concentrations of commercial disinfectants based on peracetic acid, hypochlorite, quaternary ammonium compounds and alkyl aminoacetate (TP-99). There were no indications of cross-resistance between disinfectants and antibiotics.

Serratia marcescens may survive and multiply in disinfecting footbaths containing TEGO 103G or alkylaminoacetate due to disinfectant resistance.

Significance and impact of the study:
Disinfecting footbaths may act as contamination sources in food factories and they should not be used without regular hygienic monitoring.