Journal : Molecular Ecology Notes , vol. 6 , p. 272–275 , 2006
International Standard Numbers
Printed : 1471-8278
Electronic : 1471-8286
Publication type : Academic article
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The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allelespecific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping