Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Summary

The two main allelic variants of the Atlantic cod (
Gadus morhua
L.) pantophysin I (
Pan
I)
locus have different frequencies within different cod stocks. The
Dra
I polymorphism
which distinguishes the two alleles can thus be used for discrimination of coastal and offshore
cod populations. We present a new method for
Pan
I genotyping using fluorescent allelespecific
duplex polymerase chain reaction (PCR). This method is more rapid, reliable and
cost-effective than the previously published method and it is not affected by DNA source
and quality. This improvement is important for studies demanding high throughput and
accuracy of
Pan
I genotyping

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DOI : doi.org/10.1111/j.1471-8286.20...