HomeResultsPublicationsDifferent length (DL) qPCR for quantification of cell killing by UV-induced DNA damage

Different length (DL) qPCR for quantification of cell killing by UV-induced DNA damage

Published 2010

Read in Norwegian

Publication details

Journal : International Journal of Environmental Research and Public Health (IJERPH) , vol. 7 , p. 3376–3381–6 , 2010

Publisher : MDPI

International Standard Numbers :
Printed : 1661-7827
Electronic : 1660-4601

Publication type : Academic article

Contributors : Rudi, Knut; Hagen, Irina; Johnsrud, Bente Carina; Skjefstad, Guro; Tryland, Ingun

Issue : 9

Links :
OMTALE : http://www.mdpi.com/1660-4601/...
DOI : doi.org/10.3390/ijerph7093376

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no

Summary

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.