Published 2006

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Publication details

Journal : Methods in molecular biology , vol. 345 , p. 111–117–7 , 2006

Publisher : Humana Press

International Standard Numbers :
Printed : 1064-3745
Electronic : 1940-6029

Publication type : Academic article

Contributors : Rudi, Knut; Zimonja, Monika; Skånseng, Beate

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Kjetil Aune
Chief Librarian


Quencher extension is a novel single-step closed tube real-time method to quantify single nucleotide polymorphisms (SNPs) in combination with primer extension. A probe with a 5'-reporter is single-base extended with a dideoxy nucleotide containing a quencher if the target SNP allele is present. The reaction is measured from the quenching (reduced fluorescence) of the reporter. The relative amount of a specific SNP allele is determined from the nucleotide incorporation rate in a thermocycling reaction. The quencher extension protocol presented was developed for SNP allele quantification in Listeria monocytogenes and for microbial community analyses.