Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1

Summary

Conclusion: This is the first comprehensive experimental study showing that predicted SPs in the L. plantarum genome actually are capable of driving protein secretion. The results reveal considerable variation between the SPs that is at least in part dependent on the protein that is secreted. Several SPs stand out as promising candidates for efficient secretion of heterologous proteins in L. plantarum. The results for NucA provide some hints as to the sequence-based prediction of SP functionality, but the general conclusion is that such prediction is difficult. The vector library generated in this study is based on exchangeable cassettes and provides a powerful tool for rapid experimental screening of SPs.

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DOI : doi.org/10.1186/1471-2164-10-4...
SAMMENDRAG : http://www.biomedcentral.com/1...
OMTALE : http://www.biomedcentral.com/1...