Journal : Chemometrics and Intelligent Laboratory Systems , vol. 98 , p. 10–23–14 , 2009
Publisher : Elsevier
International Standard Numbers
Printed : 0169-7439
Electronic : 1873-3239
Publication type : Academic article
Issue : 1
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Based on a recently developed spot segmentation method, we here present a new approach to modelling of individual spots in digital images, e.g. images of DNA microarrays. From the model parameter estimates and residuals we have developed an expedient approach to automatic quality assessment and identification of corrupted spots. The suggested approach to quality control is shown to give a statistically significant decrease of the variance in gene expression log-ratio estimates for three different DNA microarray datasets. (C) 2009 Published by Elsevier B.V.