Published 2006

Read in Norwegian

Publication details

Journal : Journal of Proteome Research , vol. 5 , p. 1763–1769–7 , 2006

International Standard Numbers :
Printed : 1535-3893
Electronic : 1535-3907

Publication type : Academic article

Contributors : Jia, Xiaohong; Hildrum, Kjell Ivar; Westad, Frank; Kummen, Eiliv; Aass, Laila; Hollung, Kristin

Issue : 7

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Kjetil Aune
Chief Librarian


Changes in metabolic protein levels in biopsies during the early post mortem period in the bovine longissimus thoracis muscle were investigated by 2-DE based proteome analyses. Nine NRF (Norwegian Red) dual purpose bulls were included in the study. Twenty-four proteins underwent changes between the two sampling times and were classified into two major groups: metabolic proteins and heat shock proteins. Of the metabolic proteins, 5 enzymes involved in the glycolytic pathway and the tricarboxylic acid (TCA) cycle, increased in intensities during the post mortem period. In addition, the NADP-dependent enzyme 3-hydroxyisobutyrate dehydrogenase, associated with the TCA cycle in muscle, was increased. This documents that an increased aerobic energy metabolism occurs immediately after slaughter, with the aim to replenish the ATP levels in the muscle.