Construction of vectors for inducible gene expression in Lactobacillus sakei and L. plantarum
Journal : FEMS Microbiology Letters , vol. 229 , p. 119–126–8 , 2003
Publisher : Oxford University Press
International Standard Numbers
Printed : 0378-1097
Electronic : 1574-6968
Publication type : Academic article
DOI : doi.org/10.1016/S0378-1097(03)...
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We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.