Negative-ion electrospray ionisation-mass spectrometry (ESI-MS) as a tool for analysing structural heterogeneity in kappa-carrageenan oligosaccharides

Published 2001

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Publication details

Journal : Carbohydrate Research , vol. 334 , p. 49–59–11 , 2001

Publisher : Elsevier

International Standard Numbers :
Printed : 0008-6215
Electronic : 1873-426X

Publication type : Academic article

Contributors : Ekeberg, Dag; Knutsen, Svein Halvor; Sletmoen, Marit

Links :
OMTALE : http://www.elsevier.nl/locate/...
DOI : doi.org/10.1016/S0008-6215(01)...

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Summary

Oligosaccharides, enzymically produced from kappa-carrageenan, have been investigated by electrospray ionisation mass spectrometry (ESI-MS). The technique was used without prior derivatisation of the oligosaccharide originally obtained by size exclusion chromatography (SEC). The structure of the oligosaccharides was mainly 4-sulphated neocarrabiose (A-G4S) with an increasing length ranging from di- to dodecasaccharides. However, in the larger oligosaccharides structural motivs deviating from the perfect alternating A-G4S structure were detected, i.e. (A2S-G4S). Although resulting in reduced signal intensity, samples added NaCl also gave rise to reliable mass spectra. Desulphation was induced at elevated cone voltages and in acidic or alkaline salt solutions.