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Published 2009

Read in Norwegian

Publication details

Journal : Developmental and Comparative Immunology , vol. 33 , p. 583–591 , 2009

International Standard Numbers :
Printed : 0145-305X
Electronic : 1879-0089

Publication type : Academic article

Contributors : Sperstad, Sigmund; Haug, Tor; Paulsen, Victoria; Rode, Tone Mari; Strandskog, Guro; Solem, Stein Tore; Styrvold, Olaf B.; Stensvåg, Klara

Issue : 4

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Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no

Summary

Abstract
Crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. In this study, two crustin isoforms from Hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. They show both antibacterial and antifungal activity, with highest activity against the Gram-positive bacteria Corynebacterium glutamicum. Sequencing of the transcripts showed them to have a mature peptide of 90 amino acids and differing in three positions in the mature peptide. They were named CruHa1 and CruHa2. Real-time RT-PCR revealed that they mainly are expressed in hemocytes. Screening a cDNA library detected a crustin sequence in Paralithodes camtschaticus hemocytes, coding for a mature peptide of 98 amino acids. It was named CruPc. Based on phylogenetic inference and primary structure, CruHa1 and CruHa2 were placed within the Type I group of crustins, while CruPc belongs to the Type II.