Peptides from chicken processing by-product inhibit DPP-IV and promote cellular glucose uptake: potential ingredients for T2D management

Summary

Inhibition of dipeptidyl peptidase IV (DPP-IV) and stimulation of muscle glucose uptake are two of the key
strategies for management of type-2-diabetes (T2D). In the present study, four protein hydrolysates generated
by enzymatic hydrolysis of chicken by-product, i.e., mechanical chicken deboning residue, were
evaluated for their DPP-IV inhibitory activity as well as their effect on glucose uptake by skeletal muscle
cells. The DPP-IV inhibitory assay was performed at two concentrations (1000 μg mL−1 and 10 μg mL−1)
for the crude chicken protein hydrolysates. The hydrolysate with the highest DPP-IV inhibition was
selected for preparative-scale fractionation using size-exclusion chromatography (SEC). The SEC fractions
were tested for DPP-IV inhibitory activity as well as their effect on glucose uptake and metabolic activity
of skeletal muscle cells. The muscle cells were treated with the SEC fractions and glucose uptake was
measured based on luminescence detection of 2-deoxyglucose-6-phosphate (2DG6P). A fraction with
peptides in the lower molecular weight range was shown to promote glucose uptake and to inhibit
DPP-IV. Further chromatographic fractionation followed by inhibition assaying of the most potent SEC
fraction led to isolation of five refined peptide fractions with more than 80% DPP-IV inhibition, which
were subsequently analyzed with LC-HRMS/MS. This led to identification of 14 peptides as potential
DPP-IV inhibitors from protein hydrolysates of mechanical chicken deboning residue.

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DOI : doi.org/10.1039/c8fo02450b
NVA : hdl.handle.net/11250/2593280