Quantification of 1,3-B-D-glucan from yeast added as a functional ingredient to bread
Journal : Carbohydrate Polymers , vol. 181 , p. 34–42 , 2018
Publisher : Elsevier
International Standard Numbers
Printed : 0144-8617
Electronic : 1879-1344
Publication type : Academic article
ARKIV : http://hdl.handle.net/11250/24...
DOI : doi.org/10.1016/j.carbpol.2017...
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Due to their immunomodulatory effect, 1,3-β-G from yeast are used as functional ingredients,but reliable methods for their detection in foods are lacking. We have adapted a method based on fluorescence detection with aniline blue to quantify the amount of five commercial yeast β- glucan preparations added to crisp or yeast-leavened bread. This assay detected yeast β-glucan preparations added to different breads with an average recovery of 90, 96, 99 and 105%,while one of the preparations was overestimated, with an average recovery of 157%. The presence of cereal 1,3-1,4-β-D-glucans did not interfere with assay performance. The addition of 1,3-β-G at 0.2 and 0.5 g/100 g is low compared to the recommended dose of 1,3-β-G per serving demonstrating assay sensitivity. However, more research is needed to fully understand 1,3-β-G conformation/structure on aniline blue interaction as well as the effect of baking on structure and dissolution properties of yeast β-glucans.