Published 2017

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Publication details

Journal : Vibrational Spectroscopy , vol. 89 , p. 113–122 , 2017

International Standard Numbers :
Printed : 0924-2031
Electronic : 1873-3697

Publication type : Academic article

Contributors : Aguilar-Hernández, Iris; Afseth, Nils Kristian; López-Luke, Tzarara; Contreras-Torres, Flavio F.; Wold, Jens Petter; Ornelas-Soto, Nancy

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Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no

Summary

Surface-Enhanced Raman Spectroscopy (SERS) is a powerful surface-sensitive technique to study the vibrational properties of analytes at very low levels of concentration. In particular, detection of bioactive molecules, specifically antioxidants, is an area of interest to gain insights into the reproducible and quantitative SERS-determination. In this study, SERS measurements were systematically evaluated for ferulic acid, p-coumaric acid, caffeic acid and sinapic acid. The study objective in this research was to: 1) prepare and characterize SERS-active silver colloids; 2) cluster the as-obtained colloids through Principal Component Analysis on the basis of concentration and nanoparticle size; and 3) develop a highly sensitive SERS-based method for phenolic antioxidant detection. The reliability of the proposed method was demonstrated through detection of the phenolic antioxidants evaluated at low levels of concentration. In particular, sinapic acid was evaluated for the first time, with a limit of detection of 2.5 × 10−9 M.

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