Published 2016

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Publication details

Journal : Carbohydrate Polymers , vol. 146 , p. 197–207 , 2016

Publisher : Elsevier

International Standard Numbers :
Printed : 0144-8617
Electronic : 1879-1344

Publication type : Academic article

Contributors : Nyman, Anna Armika Tussilago; Aachmann, Finn Lillelund; Rise, Frode; Ballance, Simon; Samuelsen, Anne Berit C.

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Kjetil Aune
Chief Librarian


A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC–MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671 kDa (1 → 6)-linked α-d-mannan with single and short (1 → 2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1 M NaOH extract contained two types of β-glucan; a water soluble (1 → 6)-linked β-d-glucan with single and short (1 → 3)-linked β-glucan side chains (ACcSw) and a less branched (1 → 3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1 → 6)/(1 → 3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.