Published 15.07.2015

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Publication details

Journal : Journal of Proteome Research , vol. 14 , p. 3095–3110 , Wednesday 15. July 2015

International Standard Numbers :
Printed : 1535-3893
Electronic : 1535-3907

Publication type : Academic article

Contributors : Ingerslev, Anne Krog; Karaman, Ibrahim; Bagcioglu, Murat; Kohler, Achim; Theil, Peter Kappel; Bach Knudsen, Knud Erik; Hedemann, Mette Skou

Issue : 8

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Kjetil Aune
Chief Librarian


The effects of increased intake of dietary fiber as either whole grain rye or as resistant starch compared to a typical low dietary fiber Western-style diet on the metabolomics responses were studied in gastrointestinal content and tissue, peripheral plasma, and urine using a multicompartmental nontargeted liquid chromatography–mass spectrometry (LC–MS) approach in pigs. Both unsupervised and supervised multivariate analyses were used to study differences between the intervention groups, revealing significant effects of the dietary intervention on gastrointestinal contents and urine metabolites. Consumption of a diet composed of whole grain rye flakes and enzyme treated wheat bran increased gastrointestinal content of novel sulfate- and acetic acid-conjugated oxylipins, and urinary secretion of phenolic compounds. Furthermore, potential gastrointestinal and urinary biomarkers for consumption of resistant starch, whole grain rye, and a low dietary fiber intake were detected using multiblock analysis. This study provides insight into microbial fermentation products in the gastrointestinal tract and suggests a potential role in sulfate conjugation of metabolites on the bioavailability of ingested nutrients.