Published 2001

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Publication details

Journal : Developmental and Comparative Immunology , vol. 25 , p. 313–321 , 2001

International Standard Numbers :
Printed : 0145-305X
Electronic : 1879-0089

Publication type : Academic article

Contributors : Jørgensen, Jorunn B; Johansen, Audny; Stenersen, Børge; Sommer, Ann Inger

Issue : 4

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Kjetil Aune
Chief Librarian


Unmethylated CpG dinucleotides are more frequent in the genomes of bacteria and viruses than of vertebrates. We report herein that plasmid DNA and synthetic oliogodeoxynucleotides (ODNs) containing unmethylated CpG induce production of antiviral cytokine activity in Atlantic salmon leucocytes, whereas ODNs with an inverted motif (GpC) or with methylated cytosines have nearly no stimulatory effect. The adherent cell population, representing mainly macrophages, is directly activated by CpG-ODN, while the effect on the non-adherent population is weak. Since the peak antiviral activity in ODN-stimulated leucocytes is seen after 48 h, this might indicate that the unmethylated DNA stimulates the adherent cells to produce co-stimulatory molecules, which in turn stimulates production of antiviral cytokines in the non-adherent cell population. The potent immune activation by CpG ODNs points to possible new applications as adjuvant in fish vaccines. (C) 2001 Elsevier Science Ltd. All rights reserved.