Published 2006

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Publication details

Journal : Methods in molecular biology , vol. 345 , p. 123–135 , 2006

Publisher : Humana Press

International Standard Numbers :
Printed : 1064-3745
Electronic : 1940-6029

Publication type : Academic article

Contributors : Rudi, Knut; Jakobsen, K. S.

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian


Direct DNA-based detection methods are crucial for future environmental monitoring and clinical diagnosis. We give an overview of the many variations of sample preparation approaches for bacteria for direct analyses (i.e. without culturing) in environmental and clinical samples. We cover the issues of sampling, sample preservation, separation of the microorganisms from the environmental or clinical matrix and DNA purification. Our focus is to describe both the advantages, and the disadvantages of the methods available.