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Published 2008

Read in Norwegian

Publication details

Journal : European Food Research and Technology , vol. 226 , p. 771–778 , 2008

Publisher : Springer

International Standard Numbers :
Printed : 1438-2377
Electronic : 1438-2385

Publication type : Academic article

Contributors : Mustorp, Stina L.; Engdahl-Axelsson, C.; Svensson, U.; Holck, Askild Lorentz

If you have questions about the publication, you may contact Nofima’s Chief Librarian.

Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no

Summary

Legislation requires labelling of foods
containing allergic ingredients, amongst them celery,
mustard and sesame. Here we present robust quantitative
and sensitive methods for real-time PCR detection
of celery, mustard (Sinapis alba and Brassica sp.) and
sesame in food. The development of the DNA-based
assays was part of an eVort to generate alternative
detection methods for allergens for which eVective protein-
based assays are lacking. The celery and sesame
methods were speciWc for the celery mannitol dehydrogenase
gene and the sesame allergen encoding 2S albumin
gene, respectively, when tested against a range of
plant materials. The mustard method was speciWc for
the allergen encoding sinA gene and its homologues
present in diVerent Brassica sp. All primer probe pairs
gave high ampliWcation eYciency and sensitivities
below approximately ten molecules of puriWed template
DNA. These DNA-based detection methods will
constitute supplementary and complementary methods
to the traditional protein-based methods. Laboratories
may choose diVerent analysis formats depending on
the food matrix, the availability of speciWc tests and the
performance characteristics of the tests.

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