Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.)
Publication details
Journal : Conservation Genetics , p. 747–749 , 2008
Publisher : Springer Nature
International Standard Numbers
:
Printed
:
1566-0621
Electronic
:
1572-9737
Publication type : Academic article
Issue : 9
Links
:
DOI
:
doi.org/10.1007/s10592-007-938...
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Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no
Summary
Nine polymorphic microsatellite markers were identified by screening of 2464 ESTs derived from a cDNA library of Atlantic cod (Gadus morhua L.). About 35 novel microsatellite loci were selected and characterised in 96 individual cod. Nine markers were successfully amplified with number of alleles from 3 to 18 per locus and the average heterozygosity was 0.57 in the panel examined (range 0.29-0.86). All loci followed the Hardy-Weinberg expectation and no significant linkage disequilibrium was found in a test including all pairwise combinations. The gene identity was determined at four of the loci, confirming the associated microsatellites as Type I markers.
