A 95 Molecular Weight Distribution – Validation of the new column
Publication details
Publisher : Nofima AS
International Standard Numbers
:
Printed
:
978-82-8296-699-3
Publication type : Nofima’s reports
Series : Nofima rapportserie 36/2021
Year : 2021
Links
:
ARKIV
:
hdl.handle.net/11250/2826199
Research areas
Quality and measurement methods
If you have questions about the publication, you may contact Nofima’s Chief Librarian.
Kjetil Aune
Chief Librarian
kjetil.aune@nofima.no
Summary
In the analysis ‘A 95 Molweight Distribution of Peptides’ BioLab use the Size Exclusion Chromatography (SEC) column Superdex Peptide. After a request for a new column of the same type it was informed that the column was no longer produced. The supplier recommended a replacement column, Superdex Increase. After a validation where the two columns was compared, it was concluded that BioLab could use Superdex Increase as a replacement for Superdex Peptide. This lead to a slight increase in the uncertainty for the methode, and a restriction in the measuring area. From ‘<200 – 20 000<’ to ‘<200 – 15 000<’.