Publisher : Nofima AS
International Standard Numbers
Printed : 978-82-8296-699-3
Publication type : Nofima’s reports
Series : Nofima rapportserie 36/2021
Year : 2021
In the analysis ‘A 95 Molweight Distribution of Peptides’ BioLab use the Size Exclusion Chromatography (SEC) column Superdex Peptide. After a request for a new column of the same type it was informed that the column was no longer produced. The supplier recommended a replacement column, Superdex Increase.
After a validation where the two columns was compared, it was concluded that BioLab could use Superdex Increase as a replacement for Superdex Peptide. This lead to a slight increase in the uncertainty for the methode, and a restriction in the measuring area. From ‘<200 – 20 000<’ to ‘<200 – 15 000<’.