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Published 2015

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Publication details

Publisher : Nofima AS

International Standard Numbers :
Printed : 978-82-8296-332-9

Publication type : Nofima’s reports

Contributors : Hovde, Gunnhild; Wang-Andersen, Jarle; Asbjørnsen, Bente

Series : Nofima rapportserie 39/2015

Year : 2015


Biogenic amines are formed by microbial decarboxylation of amino acids and represent a considerable toxicological risk in some food products.

This method is intended for quantification of histamine, cadaverine and putrescine in krill meal down to 10 mg/kg, which was determined to be a reasonable quantification limit. The method uses liquid chromatography with OPA (o-Phthaldialdehyde) as derivatization reagent followed by fluorescence detection. Recovery experiments showed that the recovery of the amines is good, between 88 and 108 %, for all concentration levels (approximately 2.40-120 mg/kg). The method is fit for purpose.

This report is a continuation of Nofima report 15/2015 “Histamine in fish: Liquid chromatographic determination with post-column derivatization and fluorescence detection”, and some chapters are equivalent to report 15/2015.